FTIR Microscopy for Real-World Challenges
How To Guide
Published: June 6, 2025

Credit: Perkin Elmer
From pharmaceutical uniformity to forensic evidence, modern labs face growing complexity in sample analysis. Whether identifying microplastics, analyzing polymer layers or ensuring drug formulation consistency, precision and speed are essential. Yet, many current systems fall short under pressure.
Reliable FTIR microscopy must go beyond basic imaging – it must adapt to diverse materials, surface types and research needs.
This guide explores how advanced FTIR microscopy empowers confident analysis across real-world scenarios, offering guided workflows, sharper visualization and rapid contaminant detection.
Download this guide to explore:
- A new approach to FTIR microscopy that accelerates analysis and improves outcomes
- Approaches for visualizing polymers and verifying pharmaceutical formulations
- How to accelerate decision-making in forensic, packaging and environmental testing
Spotlight™ Aurora FTIR Microscope
SHARPER INSIGHTS.
DECISIONS.
SMART
FROM UNKNOWN TO UNDERSTOOD
Analyzing unknown or complex samples is a growing challenge—
variations in size, shape, and composition make accurate
identification difficult. Labs are under pressure to deliver faster
insights without compromising quality, all while ensuring
repeatability and reducing manual effort. At the same time, the
risk of releasing non-compliant products threatens both brand
reputation and regulatory standing.
Introducing the PerkinElmer Spotlight™ Aurora FTIR Microscope.
Designed to simplify handling of unknown samples, accelerate
contaminant identification, and ensure accurate, reproducible results.
It combines rapid automated contaminant recognition, real-time
spectral matching, and guided troubleshooting workflows to deliver
fast, actionable insights with high confidence.
With integrated compliance support, robust Attenuated Total
Reflectance (ATR) performance, and modular automation,
Spotlight Aurora streamlines analytical workflows, reducing
risk and maximizing efficiency for labs managing diverse,
challenging samples.
From Unknown To Understood
Visualize With More Clarity and Detail
Maximize Return On Investment
Cut Analysis Time With Smart Workflows
Instant Clarity, Trusted Results
uATR Sampling
Visible Imaging System
Detector Switching
Automated Aperturing and Stage Control
Advanced Optics
Transform Complexity Into Clarity
The FTIR Engine
Intelligent Software Behind Every Insight
Determining Polymer Composition
The Right Materials Make all
the Difference
Every Particle Tells a Story
Your Partner in Solving Crime
VISUALIZE WITH MORE CLARITY AND DETAIL
Quickly and efficiently identify your region of interest for IR analysis. Finding your region of interest is
now faster and easier with our novel widefield viewing system, seamlessly overlaid with high-definition
detail through advanced optics and software.
Achieve precise visual imaging of uneven and curved samples with advanced depth-focus tuning, while
high-definition objectives reveal the finest details.
From Unknown To Understood
Visualize With More Clarity and Detail
Maximize Return On Investment
Cut Analysis Time With Smart Workflows
Instant Clarity, Trusted Results
uATR Sampling
Visible Imaging System
Detector Switching
Automated Aperturing and Stage Control
Advanced Optics
Transform Complexity Into Clarity
The FTIR Engine
Intelligent Software Behind Every Insight
Determining Polymer Composition
The Right Materials Make all
the Difference
Every Particle Tells a Story
Your Partner in Solving Crime
VISUALIZE WITH MORE CLARITY AND DETAIL
Maintain consistent image quality across large sample areas with adaptive visual image correction.
Seamlessly integrate IR and visible displays using our software visualization tools. Gain enhanced
depth of field and a wider field of view with multiple visible objectives, along with superior visualization
of surface topography through bright and dark field illumination options.
From Unknown To Understood
Visualize With More Clarity and Detail
Maximize Return On Investment
Cut Analysis Time With Smart Workflows
Instant Clarity, Trusted Results
uATR Sampling
Visible Imaging System
Detector Switching
Automated Aperturing and Stage Control
Advanced Optics
Transform Complexity Into Clarity
The FTIR Engine
Intelligent Software Behind Every Insight
Determining Polymer Composition
The Right Materials Make all
the Difference
Every Particle Tells a Story
Your Partner in Solving Crime
MAXIMIZE RETURN ON INVESTMENT
The Spotlight Aurora FTIR microscope combines full FTIR capabilities with advanced IR microscopy, delivering unmatched
configurability to tackle future challenges beyond conventional microscopy systems. Easily enhance your existing PerkinElmer
FTIR with our multirange and multiple detector options, compatible with models from the past 15 years.
Benefit from high-quality FTIR/NIR
sampling for samples larger than
50 microns, ensuring no compromise on
quality or sampling convenience.
Gain flexibility with the compact and
transportable Spectrum Two FTIR that
can be quickly decoupled for out-of-lab use.
Maximize your investment with basic
models that are upgradeable to IR and
NIR hyperspectral imaging or multirange
systems and enjoy the convenience of the
new Thermoelectrically cooled Mercury
Cadmium Telluride (TE-MCT) point detector
that operates without liquid nitrogen.
From Unknown To Understood
Visualize With More Clarity and Detail
Maximize Return On Investment
Cut Analysis Time With Smart Workflows
Instant Clarity, Trusted Results
uATR Sampling
Visible Imaging System
Detector Switching
Automated Aperturing and Stage Control
Advanced Optics
Transform Complexity Into Clarity
The FTIR Engine
Intelligent Software Behind Every Insight
Determining Polymer Composition
The Right Materials Make all
the Difference
Every Particle Tells a Story
Your Partner in Solving Crime
CUT ANALYSIS TIME WITH SMART WORKFLOWS
Smart sample load
and exploration tools.
Automated
performance
validation - always
ready to operate.
Laminate
measurement
algorithms.
Auto-ATR systems
for rapid automated
measurements.
Custom analyses
that can be
chained together
into single-click
methods.
Automatic
particle finding.
Aperture optimization
for multiple
point analyses.
SETUP ANALYSIS REPORTING DATA
COLLECTION
New assisted workflows provide context-sensitive guidance, offering step-by-step support throughout your analysis.
From Unknown To Understood
Visualize With More Clarity and Detail
Maximize Return On Investment
Cut Analysis Time With Smart Workflows
Instant Clarity, Trusted Results
uATR Sampling
Visible Imaging System
Detector Switching
Automated Aperturing and Stage Control
Advanced Optics
Transform Complexity Into Clarity
The FTIR Engine
Intelligent Software Behind Every Insight
Determining Polymer Composition
The Right Materials Make all
the Difference
Every Particle Tells a Story
Your Partner in Solving Crime
INSTANT CLARITY, TRUSTED RESULTS
When analyzing extremely small samples or
areas of interest, it’s often difficult to get the
kind of data that enables better understanding
of each sample’s material properties.
Spotlight Aurora brings cutting-edge
innovation from PerkinElmer to your lab,
combining a dual-camera system, multiple
viewing modes, and flexible range options
for unmatched versatility. Built-in image
optimization delivers faster insights, while
advanced ATR technology enables highquality spectral analysis down to 10 microns
-empowering precise results across a wide
range of industries and applications.
Achieve uncompromised optical performance
with high performance detectors that
offer superior signal-to-noise ratios and
a broad spectral range, allowing you to
obtain maximum information from challenging
samples in the shortest time possible.
From Unknown To Understood
Visualize With More Clarity and Detail
Maximize Return On Investment
Cut Analysis Time With Smart Workflows
Instant Clarity, Trusted Results
uATR Sampling
Visible Imaging System
Detector Switching
Automated Aperturing and Stage Control
Advanced Optics
Transform Complexity Into Clarity
The FTIR Engine
Intelligent Software Behind Every Insight
Determining Polymer Composition
The Right Materials Make all
the Difference
Every Particle Tells a Story
Your Partner in Solving Crime
TRANSFORM COMPLEXITY INTO CLARITY
SEE SPOTLIGHT AURORA IN ACTION
From Unknown To Understood
Visualize With More Clarity and Detail
Maximize Return On Investment
Cut Analysis Time With Smart Workflows
Instant Clarity, Trusted Results
uATR Sampling
Visible Imaging System
Detector Switching
Automated Aperturing and Stage Control
Advanced Optics
Transform Complexity Into Clarity
The FTIR Engine
Intelligent Software Behind Every Insight
Determining Polymer Composition
The Right Materials Make all
the Difference
Every Particle Tells a Story
Your Partner in Solving Crime
uATR SAMPLING
VISIBLE IMAGING SYSTEM
DETECTOR SWITCHING
AUTOMATED APERTURING & STAGING CONTROL
ADVANCED OPTICS
THE FTIR ENGINE
uATR SAMPLING VISIBLE IMAGING SYSTEM
Our new ATR system is fully automatic, so you
don’t have to manually insert and realign the
ATR crystal every time you use it. The built-in
suspension support and fast-feedback pressure
sensor preserve sample integrity with delicate
samples and provides faster auto pressure
adjustment with multiple sample runs.
Unique dual camera/dual optics systems provide simple
automated switching between widefield and hi-definition views of
the sample, to simplify and accelerate region of interest location.
Full sample auto-illumination and auto-focus routines with new
dynamic field correction to flatten overall optical response across
the sample to provide improved mosaicing of hi definition images
for more complete sample visualization. New depth tuning
routines provide improved sample imaging over irregular, sloped
or curved surfaces deliver more accurate visualization.
From Unknown To Understood
Visualize With More Clarity and Detail
Maximize Return On Investment
Cut Analysis Time With Smart Workflows
Instant Clarity, Trusted Results
uATR Sampling
Visible Imaging System
Detector Switching
Automated Aperturing and Stage Control
Advanced Optics
Transform Complexity Into Clarity
The FTIR Engine
Intelligent Software Behind Every Insight
Determining Polymer Composition
The Right Materials Make all
the Difference
Every Particle Tells a Story
Your Partner in Solving Crime
DETECTOR SWITCHING
Our 3-site detector optical system allows up to 3 microscope
detectors to be installed simultaneously with simple motorized
beam switching between detectors. You can use a lower
sensitivity temperature-controlled MCT detector for everyday
work and larger sample regions but switch to the more sensitive
liquid nitrogen cooled MCT when the sample demands
investigation of the smallest areas (e.g. less than 20 microns)
thus saving the time and expense of using liquid nitrogen for
all measurements - using only as needed. The system is fully
configurable, and can be changed as your microscope and
imaging needs change.
From Unknown To Understood
Visualize With More Clarity and Detail
Maximize Return On Investment
Cut Analysis Time With Smart Workflows
Instant Clarity, Trusted Results
uATR Sampling
Visible Imaging System
Detector Switching
Automated Aperturing and Stage Control
Advanced Optics
Transform Complexity Into Clarity
The FTIR Engine
Intelligent Software Behind Every Insight
Determining Polymer Composition
The Right Materials Make all
the Difference
Every Particle Tells a Story
Your Partner in Solving Crime
AUTOMATED APERTURING AND STAGE CONTROL
Our fully automated IR apertures have
set the standard for automated IR
microscopy and are now incorporated
into particle finding and laminate
workflows which automatically set the
optimum aperture size to get the best
quality IR data.
The fully automated microscope sample
stage features a lower focusing optic
with reciprocal movement, eliminating
the need to remove the lower optics.
This maximizes workspace and
reduces setup time for larger samples
and accessories.
From Unknown To Understood
Visualize With More Clarity and Detail
Maximize Return On Investment
Cut Analysis Time With Smart Workflows
Instant Clarity, Trusted Results
uATR Sampling
Visible Imaging System
Detector Switching
Automated Aperturing and Stage Control
Advanced Optics
Transform Complexity Into Clarity
The FTIR Engine
Intelligent Software Behind Every Insight
Determining Polymer Composition
The Right Materials Make all
the Difference
Every Particle Tells a Story
Your Partner in Solving Crime
ADVANCED OPTICS
Designed from the ground up for IR
performance, our advanced microscope
optics are perfectly matched to our FTIR
optical engine, with use of aspheric optics
to provide the highest quality image. For
samples requiring ultra clean environments
due to risk of airborne contamination,
an optional sample purge enclosure is
available to effectively enclose the entire
sample and sample stage space and
optical path for measurement.
Auto-recognized sample holder plates
can guide the control system to ensure
consistent sample loading with standard
sample holders or Perkinelmer microscope
accessories - this ensures reproducible, safe
movements of the stage and faster setup.
From Unknown To Understood
Visualize With More Clarity and Detail
Maximize Return On Investment
Cut Analysis Time With Smart Workflows
Instant Clarity, Trusted Results
uATR Sampling
Visible Imaging System
Detector Switching
Automated Aperturing and Stage Control
Advanced Optics
Transform Complexity Into Clarity
The FTIR Engine
Intelligent Software Behind Every Insight
Determining Polymer Composition
The Right Materials Make all
the Difference
Every Particle Tells a Story
Your Partner in Solving Crime
THE FTIR ENGINE
The key to robust, high performance IR
microscopy - our system is loaded with a
range of advanced innovations designed to
provide optimal performance from whatever
configuration you choose, including a fifthgeneration, fixed mirror-pair Dynascan™
interferometer that’s immune to the effects
of tilt and shear and requires no dynamic
alignment mechanisms.
And our proven electronic source hotspot
stabilization increases measurement stability
by maintaining the IR alignment stability over
time and extends source longevity.
From Unknown To Understood
Visualize With More Clarity and Detail
Maximize Return On Investment
Cut Analysis Time With Smart Workflows
Instant Clarity, Trusted Results
uATR Sampling
Visible Imaging System
Detector Switching
Automated Aperturing and Stage Control
Advanced Optics
Transform Complexity Into Clarity
The FTIR Engine
Intelligent Software Behind Every Insight
Determining Polymer Composition
The Right Materials Make all
the Difference
Every Particle Tells a Story
Your Partner in Solving Crime
INTELLIGENT SOFTWARE BEHIND EVERY INSIGHT
The Spotlight Aurora system is powered by the latest Spectrum IR software - delivering a unified, intuitive platform that simplifies
everything from setup to reporting. With built-in automation, smart imaging, and guided workflows, it empowers users to get
accurate, reproducible results faster and with less effort.
With 21 CFR Part 11 compliant software, our latest version provides everything from automated setup to complete characterization
– in record time.
Complete Workflow Automation:Extend macros to include microscope setup, data collection, and reporting.
Configurable Validation RoutinesOne-Click Smart Routines: Speed instrument performance validation tests, so you’re always
ready for operation.
Assisted Workflows: Step-by-step guidance for tasks requiring manual adjustments, like illumination tuning.
Smart Image Analysis: Automatically find particles, boundaries, and features for fast, accurate targeting.
Built-in Validation & Checks: Automated system performance checks with auto-recognized validation holders.
Customizable & Secure: Tailor the interface to your workflow; deploy with Standard or Enhanced Security (ES) options.
From Unknown To Understood
Visualize With More Clarity and Detail
Maximize Return On Investment
Cut Analysis Time With Smart Workflows
Instant Clarity, Trusted Results
uATR Sampling
Visible Imaging System
Detector Switching
Automated Aperturing and Stage Control
Advanced Optics
Transform Complexity Into Clarity
The FTIR Engine
Intelligent Software Behind Every Insight
Determining Polymer Composition
The Right Materials Make all
the Difference
Every Particle Tells a Story
Your Partner in Solving Crime
DETERMINING POLYMER COMPOSITION
Our Spotlight Aurora IR microscopy
systems streamline your analytical testing,
providing fast and accurate chemical
composition visualization for products
like food packaging and consumer goods.
Spotlight Aurora significantly boosts
lab efficiency by streamlining visual
exploration and automating contaminant
identification -freeing up valuable resources
and accelerating decision-making without
compromising accuracy.
Detect material defects
Identify and characterize polymer layers
Analyze compositions
Troubleshoot efficiently to accelerate
product development
From Unknown To Understood
Visualize With More Clarity and Detail
Maximize Return On Investment
Cut Analysis Time With Smart Workflows
Instant Clarity, Trusted Results
uATR Sampling
Visible Imaging System
Detector Switching
Automated Aperturing and Stage Control
Advanced Optics
Transform Complexity Into Clarity
The FTIR Engine
Intelligent Software Behind Every Insight
Determining Polymer Composition
The Right Materials Make all
the Difference
Every Particle Tells a Story
Your Partner in Solving Crime
THE RIGHT MATERIALS MAKE ALL THE DIFFERENCE
In pharmaceuticals, precision is everything
- from consistent Active Pharmaceutical
Ingredient (API) dispersion in tablets to
accurate characterization of biosimilars.
That’s why leading drug developers rely
on advanced materials analysis to ensure
product quality, safety, and regulatory
compliance. Our Spotlight Aurora IR
microscope systems scan and automatically
detect impurities in the visible image of a
sample and collect the IR spectrum for rapid
material identification.
Spotlight Aurora IR microscope systems
automatically scan pharmaceutical samples,
detect impurities or inconsistencies in
the visible image, and collect high-quality
IR spectra for rapid, reliable chemical
identification - supporting confident decisions
in drug development, tablet uniformity testing,
and biosimilar bio-characterization.
From Unknown To Understood
Visualize With More Clarity and Detail
Maximize Return On Investment
Cut Analysis Time With Smart Workflows
Instant Clarity, Trusted Results
uATR Sampling
Visible Imaging System
Detector Switching
Automated Aperturing and Stage Control
Advanced Optics
Transform Complexity Into Clarity
The FTIR Engine
Intelligent Software Behind Every Insight
Determining Polymer Composition
The Right Materials Make all
the Difference
Every Particle Tells a Story
Your Partner in Solving Crime
EVERY PARTICLE TELLS A STORY
When it comes to identifying
microplastics, our Spotlight systems are
commonly used in laboratories across the
globe. Scientists have begun developing
routine methods to characterize
microplastic particle samples down to
10 microns or less.
Our Spotlight Aurora system provides
the ability to perform both automated
single-particle analysis and IR imaging,
allowing every particle to be identified
and characterized.
From Unknown To Understood
Visualize With More Clarity and Detail
Maximize Return On Investment
Cut Analysis Time With Smart Workflows
Instant Clarity, Trusted Results
uATR Sampling
Visible Imaging System
Detector Switching
Automated Aperturing and Stage Control
Advanced Optics
Transform Complexity Into Clarity
The FTIR Engine
Intelligent Software Behind Every Insight
Determining Polymer Composition
The Right Materials Make all
the Difference
Every Particle Tells a Story
Your Partner in Solving Crime
YOUR PARTNER IN SOLVING CRIME
IR microscopy is an important technique
for evidence analysis at the scene of a
crime. Samples including single fibers,
paint chips, and other residues come
in many different shapes and sizes,
and adaptable sampling and analysis
methods – coupled with dependable
performance – are keys to success.
Spotlight Aurora systems with microand macro-sampling enable you to
choose the optimum method for your
unique sample. This ensures dependable
results, every time
From Unknown To Understood
Visualize With More Clarity and Detail
Maximize Return On Investment
Cut Analysis Time With Smart Workflows
Instant Clarity, Trusted Results
uATR Sampling
Visible Imaging System
Detector Switching
Automated Aperturing and Stage Control
Advanced Optics
Transform Complexity Into Clarity
The FTIR Engine
Intelligent Software Behind Every Insight
Determining Polymer Composition
The Right Materials Make all
the Difference
Every Particle Tells a Story
Your Partner in Solving Crime
For more information visit www.perkinelmer.com/spotlightaurora
PerkinElmer U.S. LLC
710 Bridgeport Ave.
Shelton, CT 06484-4794 USA
(+1) 855-726-9377
www.perkinelmer.com
For a complete listing of our global offices, visit www.perkinelmer.com/ContactUs
Copyright ©2025, PerkinElmer U.S. LLC. All rights reserved. PerkinElmer® is a registered trademark of PerkinElmer U.S. LLC. All other trademarks are the property of their respective owners.
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