Quick Differentiation Kits for iPSC Derived Neurons

Amsbio announces an innovative range of Induced Pluripotent Stem Cell (iPSC) kits for researchers requiring a rapid, reproducible, and scalable means of generating specific neural cell types without sacrificing cell purity.

These kits use simple, highly reproducible protocols to ensure experimental consistency. These streamlined protocols allow researchers to efficiently differentiate iPSCs into target neural cells within just 1-2 weeks. Scientists can now produce neurons as needed, ensuring greater flexibility and control in design of disease modelling, regenerative medicine, and drug screening experiments. Avoiding use of animal-derived components, these kits support human-relevant research without reliance on animal models. These easy-to-use kits produce differentiated cells with no genetic footprint left behind, via a proprietary non-integrating transcription factor-based approach. Users can choose from Sendai virus or synthetic mRNA formats. Alternatively, for convenience of the user, diseased and control cell lines that have already been differentiated are available for all the below mentioned neural cell types.

Created using human iPS cells made under license from The California Institute for Regenerative Medicine (CIRM), the Quick-Neuron™ Excitatory differentiation kit sets a new benchmark for producing highly pure populations of excitatory/glutamatergic neurons.  These cells express neuronal markers (pan-neuronal marker TUBB3 and glutamatergic neuron-specific marker vGLUT1) and display typical neurite outgrowth. Furthermore, patch clamp experiments showed these neurons generate spontaneous action potentials.

The Quick-Neuron™ Dopaminergic Differentiation kit uses synthetic mRNA to enable rapid, reproducible differentiation of iPSCs into highly pure dopaminergic neuron populations. Similarly, the new Quick-Neuron™ GABAergic Differentiation Kit enables easy and efficient differentiation of your iPS cell line into GABAergic neurons in just 10 days using synthetic mRNA. Amsbio also offers a range of high-quality human iPSC-derived GABAergic neurons.  These cells express neuronal markers (pan-neuronal marker TUBB3 and dopaminergic neuron-specific marker TH) and display typical neurite outgrowth.

This exciting new range also includes Quick-Neuron™ Cholinergic Differentiation Kits for rapid, easy, and efficient differentiation of iPS cells into cholinergic neurons. The cholinergic neurons have been validated for expression of neuronal markers (pan-neuronal marker TUBB3 and cholinergic neuron-specific marker ChAT) and display typical neurite outgrowth.